This briefing provides an initial assessment of the devastating impacts of the abrupt and arbitrary termination of U.S. foreign assistance to thousands of life-saving humanitarian, health, and human rights initiatives globally in response to the executive order ‘Reevaluating and realigning United States Foreign Aid’ issued by President Donald Trump on 20 January 2025. Amnesty International warns that chaotic cuts to life‑saving humanitarian, health, and human rights initiatives have put millions of lives at risk, undermined cooperation at the heart of the multilateral system, and exacerbated harms against vulnerable populations globally. Based on case studies from 12 countries, the report shows how the sudden stoppage of aid has endangered rights to life and health and forced more people to flee their homes.